Research in the Hileman lab is highly integrative and involves phylogenetic, morphological, genomic, gene expression and gene functional analyses to address fundamental questions including:
  • What are the genetic changes that are responsible for evolutionary transitions in floral form? 
  • To what extent is parallel trait evolution the result of parallel genetic changes?
  • How are developmental networks conserved and in what ways have they diversified during flowering plant evolution? Especially following gene duplication and gene loss events.
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We study conservation/diversification of the CYCLOIDEA-dependent flower symmetry developmental program across eudicots and monocots. We are particularly interested in how evolutionary changes in the program shape flower diversity.
Current projects focus on:
  • determining the origins of the program from non-flower symmetry function.
  • developing the emerging model species, Mimulus lewisii (pictured), to expand our knowledge of the symmetry program.
 


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​Relevant publications:
  • Sengupta, A., Hileman, L.C. 2022. A CYC–RAD–DIV–DRIF interaction likely pre-dates the origin of floral monosymmetry in Lamiales. EvoDevo 13, 3. doi: 10.1186/s13227-021-00187-w
  • Sengupta, A., Hileman, L.C. 2018. Novel traits, flower symmetry, and transcriptional autoregulation: new hypotheses from bioinformatic and experimental data. Frontiers in Plant Science 9:1561 doi: 10.3389/fpls.2018.01561.
  • Zhong, J., Preston, J.C., Hileman, L.C., Kellogg, E.A. 2017. Repeated and diverse losses of corolla bilateral symmetry in the Lamiaceae. Annals of Botany 119:21211-1223 doi: 10.1093/aob/mcx012. 
  • Preston, J.C., Barnett, L.L., Kost, M.A., Oborny, N.J., Hileman, L.C. 2014. Optimization of virus-induced gene silencing to facilitate evo-devo studies in the emerging model species Mimulus guttatus DC. (Phrymaceae). Annals of the Missouri Botanical Garden 99:301-312.  doi: 10.3417/2010120.
  • Hileman, L.C. 2014. Trends in flower symmetry evolution revealed through phylogenetic and developmental genetic advances. Philosophical Transactions of the Royal Society B 369:20130348.  doi: 10.1098/rstb.2013.0348.
  • Hileman, L.C. 2014. Bilateral flower symmetry – how, when and why? Current Opinion in Plant Biology 17:146-152.  doi: 10.1016/j.pbi.2013.12.002.
  • Preston, J.C., Hileman, L.C. 2012. Parallel evolution of TCP and B-class genes in Commelinaceae flower bilateral symmetry. EvoDevo 3:6. doi: 10.1186/2041-9139-3-6.
  • Preston, J.C., Martinez, C.C., and Hileman, L.C. 2011. Gradual disintegration of the floral symmetry gene network is implicated in the evolution of a wind-pollination syndrome. Proceedings of the National Academy of Sciences, USA 108(6):2343-2348.  doi: 10.1073/pnas.1011361108.
  • Preston, J.C., Kost, M.A., and Hileman, L.C. 2009. Conservation and diversification of the symmetry developmental program among close relatives of snapdragon with divergent floral morphologies. New Phytologist 182(3):751-762.  doi: 10.1111/j.1469-8137.2009.02794.x.
  • Preston, J.C., and Hileman, L.C. 2009. Developmental genetics of floral symmetry evolution. Trends in Plant Science 14(3):147-154.  doi: 10.1016/j.tplants.2008.12.005.
  • Hileman, L.C., Kramer, E.M., and Baum, D.A. 2003. Differential regulation of symmetry genes and the evolution of floral morphologies. Proceedings of the National Academy of Sciences, USA 100(22):12814-12819.  doi: 10.1073/pnas.1835725100.
  • Hileman, L.C., and Baum, D.A. 2003.Why do paralogs persist? Molecular evolution of CYCLOIDEA and related floral symmetry genes in Antirrhineae (Veronicaceae). Molecular Biology and Evolution 20(4):591-600.  doi: 10.1093/molbev/msg063.
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We study the evolution of floral traits in Penstemon, the largest North American flowering plant genus. During Penstemon diversification, species with flowers adapted to hummingbird pollination have evolved multiple times from the ancestral condition of bee-adaptation. This parallel evolution of hummingbird adaptation provides a framework for addressing a number of exciting evolutionary and genetic questions.
Current projects focus on:
  • uncovering phylogenetic patterns of pollination syndrome trait evolution.
  • discovering whether parallel trait evolution results from parallel developmental and genetic processes.
  • studying how floral trait interactions (epistasis), and the genetic architecture of floral trait differences, constrain the way in which floral syndromes evolve.
  • building genetic and genomic resources to advance Penstemon as a model species for ecological and evolutionary research.
 
Our work in Penstemon is in collaboration with Dr. Mark Rausher (Duke University), and Dr. Carolyn Wessinger (University of South Carolina), and is funded by NSF grant: DEB-1624043
 
Relevant publications:
  • Wessinger, C.A., Rausher, M.D., Hileman, L.C. 2019. Adaptation to hummingbird pollination is associated with reduced diversification in Penstemon. Evolution Letters 3(5):521-533 doi: 10.1002/evl3.130
  • Katzer, A.M., Wessinger, C.A., Hileman, L.C. 2019. Nectary size is a pollination syndrome trait in Penstemon. New Phytologist 223:377-384 doi: 10.1111/nph.15769
  • Wessinger, C.A., Kelly, J.K., Jiang, P. Rausher, M.D., Hileman, L.C. 2018. SNP-skimming: A fast approach to map loci generating quantitative variation in natural populations. Molecular Ecology Resources 18. doi: 10.1111/1755-0998.12930
  • Wessinger, C.A., Hileman, L.C. 2016. Accessibility, constraint, and repetition in adaptive floral evolution. Developmental Biology 419:175-183. doi: 10.1016/j.ydbio.2016.05.003.
  • Wessinger, C.A., Freeman, C.C., Mort, M.E., Rausher, M.D., Hileman, L.C. 2016. Multiplexed shotgun genotyping resolves species relationships within the recently radiated North American genus Penstemon. American Journal of Botany 103:912-922. doi: 10.3732/ajb.1500519.
  • Wessinger, C.A., Hileman, L.C.*, Rausher, M.D.* 2014. Identification of major QTLs underlying floral pollination syndrome divergence in Penstemon. Philosophical Transactions of the Royal Society B 369:20130349.  doi: 10.1098/rstb.2013.0349.